The purpose of this study was to examine whether or whether the serum acid and alkaline phosphate levels of the snake-headed Murral, Channa punctatus, would change after being exposed to an aqueous extract of the latex that is produced by the Euphoria tirucalli plant. Fishes were subjected to two sub-lethal concentrations (20 percent and 40 percent of 30h-LC50) of freshly made aqueous extract of Euphorbia tirucalli for 30 hours and 60 hours, as well as in a control group. This was done in addition to the standard protocol. Serum levels of acid and alkaline phosphate were found to have increased with increasing sub-lethal concentrations and the amount of time the animals were exposed to an aqueous extract of Euphoria tirucalli plant latex. This was observed in both of the experimental groups (20 percent and 40 percent of 30h-LC50 sub lethal concentrations). Both the dose and the amount of time passed had an effect on the alterations in acid and alkaline phosphate. For the purpose of recuperation, experimental fishes were moved to their natural environment and maintained there for one week. The levels of acid and alkaline phosphate in fishes showed signs of recovery after a withdrawal period of seven days, going from extremely high values to low values. According to the findings of this study, the unusually high values of serum acid and alkaline phosphate in Channa punctatus are caused by the latex of the Euphorbia tirucalli plant.
Keywords: Aqueous extract, serum acid phosphate, serum alkaline phosphate, Euphorbia tirucalli, and Channa punctatus etc.