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Comparative total protein content and protein band analysis in the serum of channa punctatus, labeo rohita and wallago attu

https://doi.org/10.51514/JSTR.6.1.2024.12-20

Shalini Singh*, Alok Yadav, Zubaida Khatoon, Harikesh Kumar Singh, Nassolo Margret, Mohammad Waseem, Hadiya Husain

ABSTRACT

Fishes are cold blooded aquatic vertebrates and they are low fat and high-quality source of proteins, lipids and micronutrients which is a very essential nutrient for the health. In addition to proteins, fish is filled with other nutrients like omega-3-fatty acids, vitamins D, minerals and so consumption of fish provides a wide range of nutrients.The difference in nutrient content in fish depends not only on the species but also depend on the quality of feeds and habitats.The study was conducted to evaluate the protein concentration in sera of fish, which are commercially important fish speciesChanna punctatus, Labeo rohita and Wallago attu. The protein concentrations have estimated in Channa =2.580µg/µl, Labeo=2.813µg/µl, andWallago=5.463µg/µl. After gel electrophoresis of sera were observed to lack protein ranging from 40 – 30kDa of Labeo and Wallago while five major bands was shown between 43kDa to 26kDa of Channa.

Keywords: Fish, Channa punctatus, Labeo rohita, Wallago attu, Protein, Gel electrophoresis.

Posted in Volume 6, Issue No. 1 (January-March 2024)

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